【摘要】 目的:对氧化葡萄糖酸杆菌生物转化乙二醇生产乙醇酸的工艺进行研究。方法:首先采用单因素试验优化了氧化葡萄糖酸杆菌生物转化乙二醇生成乙醇酸的工艺,在此基础上通过正交试验对转化工艺进行了进一步探讨。结果:氧化葡萄糖酸杆菌生物转化乙二醇生成乙醇酸的最优工艺为12.6%静息细胞、5.6%乙二醇维持pH5.84转化48h,乙二醇单体转化率可达60.886%。结论:应用氧化葡萄糖酸杆菌生物转化乙二醇生产乙醇酸的工艺的切实可行。更多还原

【Abstract】 Objective:Study on transformation technology of ethylene glycol to glycolic acid by Gluconobater oxydans.Methods:Biotransformation from ethylene glycol to glycolic acid by G.oxydans was optimized with single factor experiments and further study with orthogonal experiment.Results: the optimum conditions for ethylene glycol transformation were as follows:concentration of resting cells 12.6%,ethylene glycol 5.6%,pH 5.84, 48h.Transformation rate reached 60.886%under the optimum conditions.Conclusion:biotransformation of ethylene glycol to glycolic acid by G. oxydans is practicable.更多还原