【摘要】 研究了不同的因素对纤维素酶酶解玉米秸秆的影响,包括时间、反应温度、底物浓度、酶用量等。采用5因素和4水平进行玉米秸秆酶解正交试验。结果表明,影响玉米秸秆酶解的因素为反应温度>水解时间>底物浓度>速度>酶用量;得到纤维素酶酶解玉米秸秆的最佳条件为反应时间48h,酶解温度55℃,底物浓度20g/L,速度130r/min,酶用量为200U/g。该研究为玉米秸秆的生物利用提供了理论依据。更多还原

【Abstract】 Effects of different factors,such as treating time,temperature,concentrations of substrate and enzyme,on efficiency of enzymatic hydrolysis of corn stalk by cellulose were studied.The orthogonal test with 5 factors and 4 levels was applied.The results showed that significance of factors ordered as follows:reaction temperature>hydrolysis time>concentration of substrate>shaking speed>concentration of enzyme.The optimum conditions for degradation of corn stalk with cellulase were:reaction temperature of 55℃,hydrolysis time of 48h,concentration of substrate of 2%, shaking speed of 130r/min,enzyme concentration 200U/g.The research provided reference for biological utilization of corn stalk.更多还原