【摘要】 目的研究帕金森病(PD)患者脑脊液蛋白四连接素在脑深部电刺激(DBS)治疗前后的表达变化,了解四连接素有无可能成为DBS治疗PD的生物标记物。方法采用荧光差异凝胶电泳(DIGE)和基质辅助激光解吸电离飞行时间质谱(MALDI-TOFMS)鉴定技术分析PD患者脑脊液中四连接素和载脂蛋白A-Ⅰ在DBS治疗围手术期的表达变化,应用Westernblotting验证围手术期的变化及随访期的表达变化。结果在围手术期,DIGE分析和Westernblotting验证均显示DBS后四连接素和载脂蛋白A-Ⅰ未出现明显改变,而随访期,四连接素的表达水平显著增高,载脂蛋白A-Ⅰ的表达水平基本平稳。当DBS治疗停止后,四连接素的表达水平显著下降,载脂蛋白A-Ⅰ却无此现象。结论四连接素的表达变化与DBS治疗有关,有可能成为DBS治疗PD的生物标记物。更多还原

【Abstract】 Objective To know whether or not tetranectin in cerebrospinal fluid (CSF) might serve as a potential biomarker in assessing and following therapeutic efficacy of deep brain stimulation (DBS) in Parkinson disease (PD) patients.Methods Two-dimensional difference gel electrophoresis (DIGE) technique,in combination with matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS),was used to determine the differentially expressed tetranectin and apolipoprotein A-Ⅰ (apo A-Ⅰ) during the perioperative period in the CSF of PD patients who underwent bilateral subthalamic nucleus (STN) DBS procedures.Western blotting was used to validate the data of proteomic analysis from the perioperative period,and also to determine the exact changes of these proteins in follow-up period.Results DIGE and Western blotting analysis both showed that the expression levels of tetranectin and apo A-Ⅰ were not significantly changed in perioperative period.In follow-up period,the expression level of apo A-Ⅰ remained stable whereas tetranectin was significantly increased,and interestingly,while the DBS was ceased in one patient because of suicide attempt,the level of tetranectin was significantly decreased.These changes of tetranectin paralleled the observed clinical effects of DBS.Conclusions Our preliminary results suggest that the variation of the expression level of tetranectin is related to the STN DBS,and tetranectin in CSF might thereby serve as a potential biomarker for DBS.更多还原