【摘要】 目的研究左卡尼汀对特发性弱精子症精子质量及蛋白激酶CK2活性的影响,从而探讨其可能的作用机制。方法利用计算机辅助精子分析(CASA)系统对35例特发性弱精子症患者接受左卡尼汀(1g/次,tid)治疗前、治疗3个月后和15例健康男性的精液进行分析;提取精子总蛋白,利用同位素掺入法检测精子蛋白激酶CK2活性。结果左卡尼汀能显著提高特发性弱精子症患者精子的活动率、前向性运动精子百分率(a+b级)、曲线运动速度(VCL)、直线运动速度(VSL)和平均路径运动速度(VAP)。左卡尼汀治疗前CK2活性每分钟计算值为1445±431,治疗后为2209±502,治疗后精液CK2活性明显升高(P<0.05)。治疗前后CK2活性变化与治疗前后精子活动率(r=0.45,P<0.05)、a+b级(r=0.58,P<0.01)、VCL(r=0.43,P<0.05)、VSL(r=0.40,P<0.05)、VAP(r=0.41,P<0.05)变化均呈正相关性。结论左卡尼汀能显著提高特发性弱精子症精子质量,其作用机制可能是增加了蛋白激酶CK2活性。更多还原

【Abstract】 Objective To study the effects of Levocarnitine on sperm quality and activity of protein kinase CK2 in patients with idiopathic asthenozoospermia,and further explore the possible molecular mechanisms.Methods Sperms,obtained from 35 idiopathic asthenozoospermia patients before and after treated with Levocarnitine(1 g/time,tid) for 3 months and 15 healthy fertile men,were analyzed by computer-aid semen analysis(CASA) system.In addition,protein was extracted from those sperm samples and the CK2 activity was assayed by detecting incorporation of 32P of [γ-32P]ATP into the substrate.Results The sperm in idiopathic asthenozoospermia patients treated with Levocarnitine showed a significant increase in sperm progressive motility,grade a+b sperm percent,curvilinear velocity(VCL),straight-line velocity(VSL) and average path velocity(VAP).CK2 activity in idiopathic asthenozoospermia patients after treated with Levocarnitine [(2209±502)cpm(count per minute)] increased significantly than that before treatment [(1445±431)cpm](P<0.05).Furthermore,the change of CK2 activity by treated with Levocarnitine was positively correlated with the change of sperm progressive motility(r=0.45,P<0.05),grade a+b sperm percent(r=0.58,P<0.01),VCL(r=0.43,P<0.05),VSL(r=0.40,P<0.05) and VAP(r=0.41,P<0.05).Conclusions Levocarnitine could increase significantly sperm quality of idiopathic asthenozoospermia patients,which may be through enhancing the activity of CK2.更多还原