【摘要】 目的研究皮动蛋白基因在结直肠组织中的表达,分析其与临床病理因素及肿瘤侵袭性转移和预后的关系。方法采用免疫组化ELIVISION二步法,检测皮动蛋白基因在27例正常黏膜组织,13例结肠腺瘤组织及80例结直肠癌组织中的表达。结果皮动蛋白表达在结直肠癌中明显高于结肠腺瘤和正常黏膜组织,正常黏膜中表达率为11.1%,结肠腺瘤中表达率为76.9%,结直肠癌中皮动蛋白阳性表达率为82.5%,组间差异均具有统计学意义(P<0.01);皮动蛋白阳性表达在低中分化癌中均显著高于高分化癌(P<0.01);有淋巴结转移组明显高于无淋巴结转移组(P<0.01);TNM分期Ⅲ～Ⅳ期组中表达显著高于Ⅰ期组(P<0.01);同时皮动蛋白的表达水平与肿瘤浸润深度、5年生存率有显著相关性(P<0.01);与性别、年龄、部位、肿瘤大小、病理类型等无关(P>0.05)。结论皮动蛋白基因的高表达与肿瘤细胞的侵袭性转移性有关,可以被用作判断结直肠癌预后的一个标志。皮动蛋白的高表达不仅可以判断结肠癌的预后而且对诊断癌前易感病变具有重要参考价值,同时提示可能在大肠腺瘤的形成中起重要作用。更多还原

【Abstract】 Objective To study the expression of Cortactin in colorectal tissues,and investigate the relationship between the expression of Cortactin and clinical pathological factors,neoplasm invasion and metastasis and prognosis.Methods Immunohistochemical ELIVISION two-step method was used to detect the expression of Cortactin in 80 cases of colorectal carcinoma,13 cases of colorectal adenoma and 27 cases of normal colorectal tissues.Results The expression of Cortactin in colorectal carcinoma was significantly higher than that in colorectal adenoma and normal colorectal tissues.The positive expression rate of Cortactin was 11.1% in normal colorectal tissue group,76.9% in colorectal adenoma group and 82.5% in colorectal carcinoma group,and the diifference between every group was statistically significant(P<0.01).Within the colorectal carcinoma tissues,the positive expression rate of Cortactin in moderate and poorly differentiated carcinoma was significantly higher than that in well-differentiated carcinoma respectively(P<0.01).The expression of Cortactin was significantly associated with lymph node metastasis(P<0.01).And there was also higher expression of Cortactin in the Ⅲ-Ⅳ stage than that in theⅠstage,and the difference was significant(P<0.01).In addition,the expression of Cortactin was significantly associated with 5-year survival rate and depth of tumor invasion(P<0.01).There was no relationship between Cortactin protein expression and gender,age,location,tumor size and pathological typer(P>0.05).Conclusions The high expression of Cortactin protein was associated with neoplasm invasion and metastasis,and Cortactin could be used as an independent prognostic biomarker for the colorectal carcinoma patients.The high Cortactin protein expression could not only estimate the prognosis of colon cancer,but also had the important reference value to diagnose the precancerous susceptibility lesions,and suggested that Cortactin possibly played an important role in colorectal adenoma formation.更多还原