【摘要】 目的探讨人参二醇组皂苷(PDS)对内毒素休克大鼠体内血管活性物质的调节作用。方法大鼠舌下静脉注射PDS进行预治疗,10 min后注射细菌内毒素(LPS,5 mg/kg)复制感染性休克模型。实验动物随机分为对照组(C组),内毒素休克组(L组),地塞米松预治疗组(LD组),PDS预治疗组(LP组)。各组大鼠于休克24、h腹主动脉取血,硝酸还原酶法测定血清中一氧化氮合酶(NOS)活性和NO 2-/NO 3-的变化,间接反映NO的水平。于休克后4 h处死动物,称取肝组织20 mg匀浆后测血栓素B2(TXB2)及6-酮-前列腺素F1α(6-Keto-PGF1α)的含量。结果肝组织中TXB2、6-keto-PGF1α及6-Keto-PGF1α/TXB2在LP组均显著低于L组。注射内毒素2 h后L组的血清NOS和NO 2-/NO 3-明显升高,LD组和LP组NOS活性、NO 2-/NO 3-显著低于L组。结论 PDS能够降低内毒素休克大鼠TXA2、PGI2和NO的水平,改善微循环状态,起到抗休克的作用。更多还原

【Abstract】 Objective To observe the effects of Ginsen panaxadiol saponins(PDS) on vasoactive substances of endotoxin shock rats.Methods Rats were given pre-treatments by sublingual venous injection of PDS and were injected lipopolysaccharide(LPS,5 mg/kg) 10 min later to generate the septic shock models.Experimental rats were randomly divided into LPS,LPS+dexamethasone(Dex),LPS+PDS(45,90 mg/kg) and control groups respectively.The shocked rats of different groups rats were drawn blood from abdominal aortas 2 and 4 h after shock to measure the changes of NOS and NO-2/NO-3.Animals were taken liver(20 mg) homogenate and measure PGF1α and TXB2 4 h after shock.Results The levels of PGF1α,TXB2 and 6-Keto-PGF1α/TXB2 were decreased obviously than those of LPS group.NOS and NO-2/NO-3 were increased obviously after rats were injected LPS.NOS and NO-2/NO-3 of LPS+Dex,LPS+PDS groups were lower than those of LPS group.The blood viscosity of LP group was obviously lower than that of LPS group.Conclusions PDS can improve the microcirculation state of LPS-shock rats by decreasing the levels of TXB2,PGF1α and NO.更多还原