【摘要】 目的探讨针灸对2型糖尿病胰岛β细胞凋亡的影响及作用机制。方法将造模成功的大鼠按随机化原则分为针刺组、用药组、安慰剂组,同时将普食大鼠作为正常对照组,每组各10只。针刺组大鼠取"后三里"(相当于足三里)、内庭和胰俞穴给予针刺治疗,用药组用罗格列酮(0.2 mg/kg体重)灌胃,安慰剂组用生理盐水(2 ml/kg体重)灌胃,均1次/d,连续4 w。观察治疗前后空腹血糖变化;实验结束时,HE染色观察大鼠胰腺组织学改变,免疫组化标记胰岛β细胞,末端脱氧核糖核酸缺口标记法(TUNEL)+免疫组化法标记凋亡的胰岛β细胞。结果针刺组、用药组胰岛β细胞凋亡率均明显低于安慰剂组(P<0.01),针刺组胰岛β细胞凋亡率有低于用药组的趋势,但无显著性差异(P>0.05)。结论针刺可有效抑制2型糖尿病大鼠胰岛β细胞的凋亡。更多还原

【Abstract】 Objective To observe the effects of acupuncture on the apoptosis of islet β-cell in type 2 diabetes mellitus(T2DM) rats.Methods Model rats were divided randomly into acupuncture,rosiglitazone control and placebo groups,each group had 10 rats.10 male normal rats were selected as normal control group.The acupuncture group rats were selected points of "Housanli","Neiting" and "Yishu" to be treated with needling.The rosiglitazone group rats were given rosiglitazone(0.2 mg/kg) intragastrically,the placebo group rats were given 0.9% NS(0.2 mg/kg) intragastrically,once daily,for 4 consecutive weeks.The change of fasting blood glucose was determined before and after treatment.The morphological change of pancrea and β-cell apoptosis were determined.Results The percentage of β-cell apoptosis of acupuncture and rosiglitazone control groups were significantly lower than that of placebo group(P=0.000),it was lower in acupuncture group than that of rosiglitazone group,but there was no significant difference between them.Conclusions Acupuncture can effectively inhibit islet β-cell apoptosis in T2DM.更多还原