【摘要】 目的探讨p38磷酸化激酶信号转导通路(MAPK)在大鼠睡眠剥夺中的作用及与炎症因子的关系。方法将40只雄性Wistar大鼠随机分为对照组、大平台组、睡眠剥夺组、抑制剂组,每组10只。采用免疫组化观察大鼠海马区白介素(IL)-1β和磷酸化p38MAK的表达,同时利用水迷宫检测大鼠认知功能。结果与对照组和大平台组对比,睡眠剥夺组IL-1β与磷酸化p38MAPK表达明显上调(P<0.05),抑制剂组显著下降(P<0.05)。认知功能评价中睡眠剥夺组明显低于对照组和大平台组(P<0.05),抑制剂治疗组明显改善(P<0.05)。结论 p38信号转导通路参与了大鼠睡眠剥夺的过程,且可引起炎症因子的释放,该作用可影响大鼠认知功能。更多还原

【Abstract】 Objective To investigate the expression of p38 MAPK and inflammatory factor after sleep deprivation in rats.Methods 40 SD male rats were randomly divided into control(NC),large platform,sleep deprivation and inhibition groups(n=10).The expressions of p38 MAPK and IL-1β were examined by immunohistochemical staining.Rat′s recognition was detected by water maze.Results Neuron cell was complete in NC group and incomplete in sleep deprivation group,large platform and inhibition group between the above two.Compared with NC and large platform groups,the expressions of p38 and IL-1β were upregulated in sleep deprivation group,and decreased in inhibition group(P<0.05).Compared with NC and large platform groups,recognition function decreased in sleep deprivation group,and improved in inhibition group.Conclusions The activation of p38 signal transferring pathway participates in sleep deprivation process and causes inflammation factor releasing,which can affect rat′s recognition function.更多还原