【摘要】 目的探讨白藜芦醇对亚急性衰老小鼠海马神经细胞的保护作用。方法 D-半乳糖颈背部皮下注射制备亚急性衰老小鼠模型,设正常对照组、对照组、高低剂量处理组和阳性对照组。第3周始,处理组分别给予白藜芦醇15、45mg/kg(低、高剂量)灌胃,对照组给予生理盐水或DMSO溶液灌胃。8周后,检测小鼠血清、脑组织中超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量。制作石蜡切片,尼氏染色观察小鼠海马CA1区形态结构,免疫组化检测P53蛋白表达,TUNEL法检测细胞凋亡。结果各组小鼠海马组织形态结构无明显变化;与对照组相比,白藜芦醇45 mg/kg体重组小鼠血清和脑组织中SOD活性明显升高,MDA含量明显下降;白藜芦醇45 mg/kg体重组小鼠海马CA1区P53蛋白表达阳性细胞较对照组小鼠减少,但各组均无明显的凋亡细胞。结论白藜芦醇能增强亚急性衰老小鼠的抗氧化功能,降低亚急性衰老小鼠海马CA1区神经元P53蛋白的表达。更多还原

【Abstract】 Objective To investigate the protective effect of resveratrol(RES) on neurons in hippocampus in subacute aging mouse.Methods The subacute aging mice were prepared by subcutaneous injection of D-galactose at the nape.From the third week,the mice were fed with resveratrol(15.45 mg / kg) by oral gavage and the control group were fed with normal saline or solution of DMSO.The superoxide dismutase(SOD) activity and malondialdehyde(MDA) level in serum and brain of mice were detected.Morphology of hippocampal CA1 area was observed through Nissil stain.The expression of P53 protein was detected by immunohistochemistry.TUNEL was used to detect apoptosis.Results Morphology of hippocampal CA1 area had no change in all groups.Compared with the control groups,SOD activity was significantly increased and MDA content was significantly decreased in serum and brain of the mice fed with 45mg/kg resveratrol.The number of P53 positive cells was less in hippocampus CA1 zone of the mice fed with 45mg/kg resveratrol than that of mices in control group.Typical apoptotic cells can not be seen in hippocampus CA1 zone of all the groups.Conclusions Resveratrol can enhance the antioxidant function of subacute aging mouse,and decrese P53 expression in hippocampal CA1 neurons in subacute aging mouse.更多还原