【摘要】 目的研究自聚肽纳米纤维支架对骨髓源性心肌干细胞(MCSCs)生长和存活的作用。方法设计和固相合成自聚肽,扫描电镜和原子力显微镜下观察其自组装后的形态结构和与MCSCs的相互关系;通过CCK-8法和免疫荧光染色检测纳米纤维支架对MCSCs增殖和分化情况。结果 1%多肽溶液自组装成直径约10 nm,长度为100~300 nm纳米纤维,并相互交织成孔径为50~200 nm的网状结构;MCSCs在纤维支架中培养1 d后,可见细胞呈长梭形,位于三维纳米纤维支架的网孔中,生长状态良好;生长曲线显示细胞在纤维支架中的增殖能力明显高于对照组;MCSCs在纤维支架中诱导培养4周时,形态和排列方式均发生明显改变。结论纤维支架与MCSCs有良好生物相容性,纤维支架有利于MCSCs的生长和定向分化。更多还原

【Abstract】 Objective To explore the effect of the self-assemble peptide nanofiber scaffold(NFS) on the proliferation and differentiation of marrow-derived cardiac stem cells(MCSCs).Methods The self-assemble peptides were designed and synthesized.The self-assemble state was viewed by AFM and SEM.The compatibility of MCSCs with self-assembling peptide nanofiber scarffolds was viewed by SEM.The effect of NFS on the growth of MCSCs was detected by CCK-8.Through cardiac-specific troponin cTnT staining,the effect of NFS was evaluated on myocardial cell differentiation of MCSCs.Results SEM showed the self-assembly of the peptides into the retiform interwoven nanofiber scaffold structure,with the stent diameter of 50 ~ 200 nm.MCSCs were seeded into the self-assemble peptide nanofibers 1 day later.Cells were detected in the network-like nanofiber,which closely linked with the nano-fibers under SEM.The viability of cells in NFS was significantly higher than that of control group.The cells in NFS contacted closely with each other,and demonstrated the same direction and arrangement.After 4 weeks’ induction,all of cells expressed cTnT.Conclusions NFS and MCSCs have good biocompatibility.The three-dimensional micro-environment of NFS is valuable for the growth and differentiation of MCSCs in vitro.更多还原