【摘要】 目的了解基质细胞衍生因子-1α(SDF-1α)在皮质发育畸形(MCD)大鼠海马中的表达,探讨其在皮质发育畸形异常局部神经回路中的作用。方法通过腹部X射线照射孕16dSD大鼠构建子代MCD模型,对30、60d对照组与实验组子鼠(P30、P60)行HE、Timm染色以检测其组织学改变,免疫组化、RT-PCR及Western-Blot检测SDF-1α的表达情况。结果①Timm染色示模型鼠海马出现苔藓纤维出芽;②免疫组化示SDF-1α在模型组海马CA1区异位神经元有表达;③RT-PCR和Western-Blot结果显示模型组P30、P60较同期的对照组表达降低。结论 SDF-1α的下调可能参与皮质发育畸形海马中异常神经回路的形成。更多还原

【Abstract】 Objective To get a message of the expression about stromal cell-derived factor-1α(SDF-1α)in the Malformations of cortical development(MCD)in rats model and investigate the effects in forming abnormal neural circuit in the MCD.Methods Sprague-Dawley pregnant rats exposed to X-irradiation on embryonic 16(E16)days can induce MCD in the offspring.We observed the histological changes of the brain with HE staining and Timm staining,and the expression of SDF-1α with immunohistochemistry,RT-PCR and Western-Blot in the pups at postnatal 30 days(P30)and postnatal 60 days(P60).Results ①Timm staining demonstrated mossy fiber sprouting was formed in the MCD model;②Immunohistochemical results showed that expression of SDF-1α appeared in the CA1 ectopic neurons of hippocampus;③From the results of RT-PCR and Western-Blot,we got a reduced expression of SDF-1α at P30 and P60 in the model group contrast to control group at the same period.Conclusion the downregulation of SDF-1α may play a role in forming abnormal neural circuit in hippocampus of MCD.更多还原