【摘要】 目的探讨野生型PTEN基因在人乳腺癌细胞系MCF-7和ZR-75-1中对表阿霉素的增敏作用。方法腺病毒介导野生型PTEN基因导入人乳腺癌细胞系MCF-7和ZR-75-1,RT-PCR检测PTEN mRNA的表达,Western blot检测转染后PTEN蛋白的表达;Ad-PTEN感染联合不同浓度的表阿霉素处理细胞,采用CCK-8法测定细胞增殖抑制率和联合效应。结果腺病毒介导的PTEN基因导入法可明显地增加细胞中PTEN基因的表达,野生型PTEN基因转染联合表阿霉素使乳腺癌细胞MCF-7对表阿霉素的敏感度增加了两倍,而使乳腺癌细胞ZR-75-1对表阿霉素的敏感度增加了十倍。结论腺病毒重组的野生型PTEN基因联合表阿霉素对人乳腺癌细胞增殖具有显著的协同抑制效应。更多还原

【Abstract】 Objective To investigate the influence of wild-type PTEN gene on chemo-sensitivity of Epirubicin in breast cancer cell lines MCF-7 and ZR-75-1.Methods The wild-type PTEN gene was transfected into human breast cancer cell line MCF-7 and ZR-75-1 by recombinant adenoviruses(Ad-PTEN).The expression of PTEN gene mRNA was analyzed by RT-PCR and the PTEN protein was identified by Western blot.The effect of Epirubicin alone or combined with Ad-PTEN on cell survival rate was measured by CCK-8 test.Results Adenovirus-mediated PTEN gene transfer system obviously increased the expression of PTEN mRNA and protein in cells.After treatment with Ad-PTEN,the chemosensitivity of breast cancer cells to Epirubicin increased about two folds in MCF-7 cells and ten folds in ZR-75-1 cells.Conclusion The synergism of Ad-PTEN to epirubicin obviously increased the cytotoxicity on breast cancer cell lines.更多还原