【摘要】 目的:探讨5-羟色胺转运体(5-HTT)基因启动子区CpG岛甲基化状态与精神分裂症Ⅰ型和Ⅱ型的关联。方法:运用特异性甲基检测PCR和直接测序法对62例精神分裂症Ⅰ型患者、38例Ⅱ型患者和50例健康被试5-HTT基因启动子区CpG岛甲基化状态进行检测。结果:三组5-HTT基因启动子区CpG岛甲基化阳性率无显著性差异;精神分裂症Ⅰ型患者5-HTT基因启动子区CpG岛内位点甲基化率显著高于精神分裂症Ⅱ型患者和健康被试组。结论:5-HTT基因启动子区CpG岛内位点高甲基化可能是精神分裂症Ⅰ型的发病机制之一。更多还原

【Abstract】 Objective: To explore the relationship between methylation status of CpG islands in the promoter region of serotonin transporter gene(5-HTT) and type Ⅰ or Ⅱ schizophrenia.Methods: Methylation Specific PCR and DNA sequencing were used to detect the methylation status of CpG islands in 62 type Ⅰ schizophrenia patients,38 type Ⅱ schizophrenia patients and 50 health subjects.Results: The methylation rates of CpG islands within the promoter region of 5-HTT of the three groups are 52%(32/62),47%(18/38) and 50%(25/50),respectively.The methylation rates of loci in CpG islands within the promoter region of 5-HTT in type Ⅰ schizophrenia group is significantly higher than those in type Ⅱ schizophrenia group and health subjects.Conclusion: The high methylation rate of loci in CpG islands within the promoter region of 5-HTT should be a possible factor to influence the nosogenesis of type Ⅰ schizophrenia.更多还原