【摘要】 目的研究鲍曼不动杆菌烧伤分离株对广谱抗生素的耐药性及所携带的广谱抗生素及消毒剂耐药基因。方法测定20株分离自烧伤患者的鲍曼不动杆菌对四环素、米诺环素、氯霉素、利福平、复方磺胺甲噁唑5种广谱抗生素的敏感性,PCR检测catB、cmlA、arr-2/3、tetA、tetB、smr-2、emrE、dfrA1、dfrA5、dfrA7、dfrA12、dfrA17、dfrB5、qacEΔ1-sul1和intI共15种基因。结果 20株细菌对5种抗生素的敏感率分别为10%、100%、0、0和5%。tetB、qacEΔ1-sul1和intI基因检出率均为95%(19/20),其余12种基因为阴性,且一株静脉导管分离株携带了上述3种基因。结论本组鲍曼不动杆菌烧伤分离株对除米诺环素外的广谱抗生素耐药严重,并携带了四环素类和消毒剂耐药基因。应规范此类抗生素在养殖业中的使用,同时采取措施防止多重耐药菌株利用静脉导管在烧伤科传播。更多还原

【Abstract】 Objective To investigate the broad-spectrum antibiotics resistance and broad-spectrum antibiotics and antiseptics resistance genes of Acinetobacter baumannii strains isolated from burned patients.Methods Susceptibilities to tetracycline,minocycline,chloramphenicol,rifampicin and trimethoprim-sulfamethoxazole were tested.Subsequently,catB,cmlA,arr-2/3,tetA,tetB,smr-2,emrE,dfrA1,dfrA5,dfrA7,dfrA12,dfrA17,dfrB5,qacEΔ1-sul1 and intI were detected by PCR.Results Susceptibilities to five broad-spectrum antibiotics were 10%,100%,0,0 and 5% respectively.19/20(95%) isolates carried tetB,qacEΔ1-sul1 and intI genes while other 12 genes were not detected.Notably,an isolate colonising a central venous catheter carried all three genes mentioned above.Conclusions Acinetobacter baumannii strains isolated from burned patients we studied had serious resistances to broad-spectrum antibiotics except minocycline and carried tetracyclines and antiseptics resistance genes.Accordingly,broad-spectrum antibiotics should be standardized utilized in aquaculture.Furthermore,measures should be carried out to prevent the dissemination of multidrug resistant strains through the central venous catheter.更多还原