【摘要】 目的:检测结直肠正常黏膜-结直肠息肉-结直肠癌中Caspase凋亡通路上相关基因的mRNA表达水平,探讨其在结直肠癌发生、发展中的作用。方法:收集了19例结直肠癌患者的肿瘤组织、86例结直肠息肉患者的息肉组织和10例正常对照的结直肠黏膜组织。采用实时荧光定量逆转录聚合酶链反应(RT-PCR)测定结直肠正常黏膜、结直肠息肉、结直肠癌中Caspase通路上基因(Caspase-2-、3-、6、-7、-8、-9和-10)的mRNA表达水平。结果:年龄、性别等人口学特征在正常对照、息肉及结直肠癌病例组间分布均衡;与正常黏膜组比较,除Caspase-3在结直肠癌中表达升高外,其它基因mRNA表达均下降,但都没有达到统计学上的显著性水平;此外,除Caspase-9外,这些Caspase基因相互间mRNA表达水平存在显著的的正相关关系。结论:未发现Caspase凋亡通路上相关基因mRNA表达水平在肿瘤形成过程中发生显著变化;Caspase通路上基因相互之间表达水平呈显著相关。更多还原

【Abstract】 Objective:To investigate mRNA expression of caspase apoptosis pathway genes in colorectal cancer,polypus and normal mucosa.Methods:Nineteen patients with colorectal cancer,86 patients with polypus and 10 normal controls were enrolled from 2008 to 2010.Fluorescence quantitative RT-PCR was performed to detect the mRNA expression of caspase apoptosis pathway genes(caspase-2,-3,-6,-7,-8,-9 and-10) in colorectal cancer,polypus and normal mucosa.Results:There were no statistically significant differences of demographic characteristics between patients with colorectal cancer,patients with polypus and normal controls.Compared with normal control group,the mRNA expression of all selected genes except for caspase-3 were lower;however,the P values did not reach statistic significance.Highly positive correlations were observed between mRNA expression of all selected genes except caspase-9.Conclusions:There are no significant changes in mRNA expression levels of caspase apoptosis pathway genes from normal mucosa to polypus to cancer.The mRNA expressions of most caspase pathway genes are highly correlated with each other.更多还原