【摘要】 目的:建立半枝莲总黄酮有效部位中4种有效成分定量分析方法。方法:采用高效液相色谱法。色谱柱为Agilent Zorbax C18(4.6 mm×250 mm,5μm),流动相:A相1%醋酸、B相CH3OH-CH3CN(80∶20),线性梯度程序洗脱,流速1 ml/min,检测波长280 nm,柱温30℃,进样量10μl。结果:半枝莲总黄酮主要有效成分为野黄芩苷(scutellarin)、异野黄芩素-8-O-葡萄糖醛酸苷(isoscutellarein-8-O-glucuronide)、异野黄芩素(isoscutellerin)和木犀草素(luteolin),其线性范围分别为0.14~11.20μg、0.03~2.40μg、0.007~0.560μg、0.027~2.160μg;平均加样回收率分别为(101.9±1.4)%、(103.5±0.6)%、(98.1±2.9)%、(100.5±2.3)%。结论:该定量分析方法专属性强、重现性好,可用于半枝莲总黄酮有效部位的质量控制。更多还原

【Abstract】 Objective: To establish a HPLC method for simultaneous determination of 4 effective components from total flavonoids of Scutellaria barbata(FSB).Methods: The HPLC method was developed on an Agilent Zorbax C18 column(4.6 mm×250 mm,5 μm).The mobile phase was composed of 1% HAc and CH3OH∶CH3CN(80∶20) with a linear gradient elution.The flow rate was 1.0 ml/min,and UV detection wave length was set at 280 nm.The column temperature was maintained at 30℃.Results: The linear range of 4 effective components(scutellarin,isoscutellarein-8-O-glucuronide,isoscutellerin and luteolin) was 0.14-11.20 μg,0.03-2.40 μg,0.007-0.560 μg and 0.027-2.160 μg,respectively.The average recovery for 4 effective components was(101.9±1.4)%,(103.5±0.6)%,(98.1±2.9)% and(100.5±2.3)%,respectively.The contents of 4 flavonoids were determined,with scutellarin 7.3%-14.3%,isoscutellarein-8-O-glucuronide 2.4%-9.3%,isoscutellerin 0.3%-0.5%,and luteolin 0.2%%-0.6%,respectively.Conclusion: The method can be used effectively to evaluate the quality of FSB.更多还原