【摘要】 [目的]探讨骨膜对骨-肌腱结合部位愈合的影响,通过实验证明骨膜促进骨-肌腱结合部位细胞增生,进而促进该部位愈合。[方法]48只18周龄新西兰大白兔随机分为2组,实验组将骨膜游离并植入兔髌骨部分切除模型中骨-肌腱结点中,对照组只进行手术,不植入骨膜。在术后4、8、12周处死动物取标本进行大体和组织学观察。[结果]大体观察可见实验组骨-肌腱结合部位愈合较早。组织学检查显示实验组术后4、8周骨-肌腱结合部组织愈合明显,以从松质骨再生和骨-肌腱愈合接点纤维软骨带的再生为特征,较对照组迅速,提示早期实验组恢复较对照组迅速。[结论]骨膜可以促进骨-肌腱结合部位细胞增生,增加细胞基质合成,促进新生骨和纤维软骨移行带形成,促进其愈合。更多还原

【Abstract】 [Objective]To explore effects of periosteum on healing of bone-tendon junction and provide experimental evidence on treating injuries of bone-tendon junction via periosteum accelerating cellular proliferation and healing. [Methods]Standard animal model of partial patellectomy in 36 mature female New Zealand white rabbits(18-weeks-old) was established and randomly classified into 2 groups.The experimental group received periosteum while the control group was only operated on.The quadriceps muscle-patella-patellar tendon(QMPPT) were harvested at 4,8,and 12 weeks postoperatively for histologic observation(standard H&E stain、Safranin’O stain and Masson strain).[Results]Gross observation found the experimental group healing faster than the control group.Histological study showed the experimental group at 4,8,and 12 weeks had more improved tissue integration,characterized by trabecular bone expansion from the remaining patellar and regeneration of fibrocartilage zone at the patellar tendon-patellar healing junction.The tissue healing in the experimental group was quicker than that in the control group.[Conclusion]Periosteum could accelerate celluar proliferation,matrix synthesis,new bone and fibrocartilage transitional zone formation and accelerate bone-tendon junction healing.更多还原