Objective To lay the foundation for the development of a uropathogenic Escherichia coli gene vaccine by constructing and identifying the eukaryotic expression vector pcDNA3.0-fimH for expression of the fimH gene from uropathogenic E.coli.Methods Gene fragments of fimH from uropathogenic E.coli 6704 were amplified by PCR.The purified PCR fragments were ligated into the pMD19-T simple vector,the positive clones were directly screened from bacteria and identified by PCR,and the PCR products were digested via d...
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