【摘要】 目的通过对眼病患者和健康人群弓形虫感染调查,探讨并分析眼病是否与弓形虫感染有关。方法采用ELISA检测160例眼病患者和160名健康人血清弓形虫IgM抗体,用PCR检测血液中弓形虫DNA。结果160例眼病患者,20例IgM抗体阳性,阳性率为12.50%;健康对照160例,9例阳性,阳性率为5.63%,差异有统计学意义(χ2=4.588,P<0.05)。眼病组中80例中渗患者IgM阳性15例,阳性率为18.75%;80例葡萄膜炎患者IgM阳性5例,阳性率为6.25%,二者差异有统计学意义(χ2=5.714,P<0.05)。健康对照组中,80名大学生3例IgM阳性,阳性率为3.75%;80名工人6例阳性,阳性率为7.50%,二者差异无统计学意义(χ2=1.060,P>0.05)。用Tox-PCR检测20例IgM阳性的眼病患者,均为阳性,健康对照组的9例IgM阳性者PCR阳性8例,二者差异有统计学意义(χ2=3.079,P<0.01)。结论眼病患者弓形虫感染率高于健康人,眼病中渗患者弓形虫感染率高于葡萄膜炎患者。因此认为某些眼病与弓形虫感染有关。更多还原

【Abstract】 Objective To investigate toxoplasmosis among patients with an eye disease and healthy people and analyze the association between eye disease and toxoplasmosis.Methods Sera were collected from 160 patients with an eye disease and 160 health people.ELISA was used to detect anti-Toxoplasma gondii IgM and PCR was used to detect DNA.Results One hundred and sixty eye patients were positive for Tox-IgM at a rate of 12.50%(20/160)and healthy people were positive at a rate of 5.63%(9/160).There was a significant difference in that rate for the two groups(χ2= 4.588,P<0.05).Eighty patients with central exudative chorioretinopathy were positive at a rate of 18.75%(15/80),and patients with uveitis were positive at a rate of 6.25%(5/80);there was a significant difference in that rate for the two groups(χ2=5.714,P<0.05).University students were positive at a rate of 3.75%(3/80) and workers were positive at a rate of 7.50%(6/80);there were no significant differences in that rate for the two groups(χ2=1.060,P>0.05).Twenty patients with an eye disease tested positive for IgM according to Tox-PCR;among healthy people,9 tested positive for IgM and 8 tested positive according to PCR.There was a significant difference in positivity for the two groups(χ2=3.079,P<0.01).Conclusion The rate at which patients with an eye disease were positive for infection with Toxoplasma gondii was higher than that for healthy people and the rate at which patients with central exudative chorioretinopathy were positive for that infection was higher than that for patients with uveitis.Therefore,some eye diseases are associated with infection with Toxoplasma gondii.更多还原