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贵州金丝桃组培快速繁育技术研究
Study on Tissue Culture Rapid Breeding Technology of Hypericum kouytcheouense
【摘要】 为了建立贵州金丝桃快繁再生体系,对贵州金丝桃进行了组织培养研究。结果表明:茎段为贵州金丝桃组织最适外植体,初代培养最适宜芽诱导的培养基配方为1/4 MS+TDZ0.5 mg/L+NAA0.01 mg/L;芽继代增殖最适宜的培养基配方为1/4 MS+ZT1.5 mg/L+NAA1.0 mg/L;最适宜壮苗生根的培养基为1/4 MS+ZT0.1 mg/L+NAA1.0 mg/L。
【Abstract】 In order to establish rapid propagation regeneration system of Hypericum kouytcheouense,the author carried out this study.The result showed that :The stem was the most suitable explant,and the most suitable culture medium for induction bud was 1/4 MS+TDZ 0.5 mg/L+NAA 0.01 mg/L at the primary culture step.At the secondary multiplication step,the most suitable culture medium formula of the bud was 1/4 MS+ZT 1.5 mg/L+NAA 1.0 mg/L,and the most suitable medium for seedling root was 1/4 MS+ZT 0.1 mg/L+NAA 1.0 mg/L.
【关键词】 贵州金丝桃;
外植体;
初代培养;
继代增殖;
生根培养;
【Key words】 Hypericum kouytcheouense; explant; primary culture; secondary multiplication; rooting training;
【Key words】 Hypericum kouytcheouense; explant; primary culture; secondary multiplication; rooting training;
【基金】 贵州省科学技术基金项目(黔科合J字[2009]2223号);贵州省科技创新能力建设项目(黔科合院所创能[2009]4008号)联合资助
- 【文献出处】 种子 ,Seed , 编辑部邮箱 ,2011年10期
- 【分类号】S567.239
- 【被引频次】3
- 【下载频次】105