【摘要】 目的探讨建立更快速、准确的痰结核分枝杆菌检测方法。方法采用涂片抗酸染色法、罗氏培养法和DNA环介导恒温扩增(LAMP)试验对结核分枝杆菌进行检测,并对其检出率进行比较。结果 LAMP试验检出率最高,罗氏培养法次之且检测时间长,抗酸染色法的检出率最低。结论 LAMP试验检测痰结核分枝杆菌具有快速、敏感、简便、特异等优点,在肺结核患者的早期诊断中将发挥重要作用。更多还原

【Abstract】 OBJECTIVE To develop method of a more rapid and accurate detection of Mycobacterium tubarculosis.METHODS Acid-fast strainin by smear,Lowenstein-Jensen culture and loop-mediated isothermal amplification(LAMP) test for detection of Mycobacterium tuberculosis,and compared their detection rate.RESULTS LAMP test the highest detection rate of Mycobacterium tuberculosis,followed by TB culture and a long time,the lowest detection rate of acid fast staining method to detect.CONCLUSION The LAMP test is rapid,sensitive,simple and specific method for the detection of Mycobacterium tuberculosis.In the early diagnosis of tuberculosis will play an important role in.更多还原