【摘要】 目的了解浙南山区产超广谱β-内酰胺酶(ESBLs)的大肠埃希菌和肺炎克雷伯菌的耐药性,为临床合理使用抗菌药物提供依据。方法采用CLSI推荐的双纸片协同试验;用头孢他啶、头孢他啶/克拉维酸和头孢噻肟、头孢噻肟/克拉维酸确证试验确认,检测医院2008年1月-2009年12月临床标本中分离出的107株产ESBLs肺炎克雷伯菌和274株产ESBLs大肠埃希菌;用K-B法进行药物敏感试验,并用上海新和实验室管理软件分析细菌分布和耐药性。结果 2008、2009年产ESBLs大肠埃希菌和肺炎克雷伯菌分别为51.6%、21.5%和47.0%、17.9%,未发现对亚胺培南耐药。结论浙南山区产ESBLs大肠埃希菌和肺炎克雷伯菌与高发地区相近,但是在浙江建立多药耐药菌监测制度后,产ESBLs的菌发生率出现下降。更多还原

【Abstract】 OBJECTIVE To study drug resistance of ESBLs-producing Klebsiella pneumoniae and Escherichia coli,to provide basis for rational application of antibiotics.METHODS Using doubt disk synergy test recommended by CLSI,which contained ceftazidime,ceftazidime/Clavulanic acid and cefotaxime,cefotaxime/clavulanic acid,A total of 107 strains of ESBLs-producing K.pneumoniae and 274 strains of ESBLs-producing E.coli isolated from clinical samples of our hospital during Jan 2008-Dec 2009 were detected.The K-B assay was used for drug sensitivity test and the Lab management software produced by shanghai Xinhe was used to analyze the distribution and drug resistance of the tow bacteria.RESULTS The detection rates of ESBLs-producing K.pneumoniae and E.coli isolated during 2008-2009 were 51.6%,21.5%,47.0% and 17.9%,respectively.No imipenem-resistant strains was discovered.CONCLUSION The incidences of ESBLs-producing K.pneumoniae and E.coli in mountainous area of Zhejiang Province are similar to that of high occurrence areas.Fortunately the rate is droppped since the multiple-drug bacteria monitoring system is established.更多还原