【摘要】 目的对浙江台州地区的产ESBLs大肠埃希菌ESBLs基因型进行分析,指导临床合理使用抗菌药物。方法超声破碎法提取细菌的β-内酰胺酶并对其进行等电聚焦电泳,然后根据pI值结果设计引物,以聚合酶链反应获得目的基因。结果 ESBLs阳性检出率29.1%,pI值与基因型结果:基因分型中,除9株(10%)未有分型外,全部为CTX-M型(81株,90%),测序结果亚型分别为:M-14(41株);16株M-14合并广谱β-内酰胺酶TEM-1;M-9(10株);M-22(8株);M-24(4株);M-27(1株);M-15(1株);pI值结果为M-14的pI值8.1(46株);18株有8.1、5.4两个pI值;8株测序结果为M-14合并广谱β-内酰胺酶TEM-1;7.9(1株);8.2(1株);8.6(5株),8.9(1株),M-9的pI值7.9,M-24,M-27的pI值8.2,M-15,M-22的pI值为8.4,9株未测出基因型2株pI值为5.4、8.1;2株pI值为5.4;2株pI值为6.2;1株pI值为8.9;2株pI值未测出。结论浙江台州地区大肠埃希菌ESBLs阳性率高并具有基因多样性。更多还原

【Abstract】 OBJECTIVE To analyze the genotypes of ESBLs producing Escherichia coli,so as to guide the rational use of antibiotics.METHODS Bacterial β-lactamase was extracted by using sonication,and then was performed the IEF,the primers were destghed by the pI values of the results and the targeted genes were obtained by polymerase chain reaction.RESULTS ESBLs positive rate was 29.1%,pI values and genotype results:genotyping,except for nine(10%) did not type,all the CTX-M type(81,90%),sequencing subtypes were:M-14(41 strains);16 M-14 extended-spectrum β-lactamase combined TEM-1;M-9(10);M-22(8);M-24(4);M-27(1);M-15(1).pI values of the results of the pI value of M-14 8.1(46);18 were 8.1,5.4 two pI values;eight sequencing results for the M-14 extended-spectrum β-lactamase combined enzyme TEM-1;7.9(1 strain);8.2(1);8.6(5),8.9(1),M-9 in the pI values of 7.9,M-24,M-27 in the pI values of 8.2,M-15,M-22 in the pI value of 8.4,9 strain genotype 2 was not measured pI values 5.4,8.1;2 pI value of 5.4;2 pI value of 6.2;a pI value of 8.9;2 pI value is not measured.CONCLUSION The positive rate of E.coli producing ESBLs in Taizhou is high and presents genetic diversity.更多还原