【摘要】 目的建立测定番石榴叶、苦瓜干和苦丁茶中槲皮素含量方法。方法采用DiamonsiLTM C18(250mm×4.6mm,5μm),以甲醇-0.5%磷酸溶液(58:42)为流动相,检测波长为372nm,流速:1.0ml/min。结果槲皮素在1.064～10.64μg/ml之间成良好线性关系(r=0.9996);番石榴叶、苦瓜干和苦丁茶的平均回收率分别为99.92%(RSD=0.4%)、102.6%(RSD%=0.7%)和102.9%(RSD%=0.1%)。结论该方法准确、灵敏,可作为槲皮素HPLC分析的一个有效的方法。更多还原

【Abstract】 Objective To determine the content of quercetin with HPLC.Methods Content was measured by DiamonsiL C18(250mm×4.6mm,5μm)column,methanol-0.5% phosphoric acid(58:42)as mobile phase,detection wave length of 372nm flow rate 1ml/min.Results Quercetin in the range of 1.064～10.64μg/ml a good linear relationship(r=0.9996),the average recovery of Guava leaf,Momordica charantiap,Kuding tea was 99.92%(RSD=0.4%),102.6%(RSD%=0.7%)and 102.9%(RSD%=0.1%),respectively.Conclusion The method is rapid,accurate and can be used for determination of quercetin by HPLC.更多还原