【摘要】 目的研究血卟啉衍生物介导的光动力(HPD-PDT)抑制胆管癌细胞生长状况及其机制。方法 MTT法用于评估人胆管癌细胞(QBC939)的生长状态;Hoechst33258染色和流式细胞术检测细胞凋亡;WesternBlotting法用于探测QBC939细胞中细胞色素C的释放情况;Caspases酶活性测定用于测定caspase-3、-8、-9的活化情况。结果 HPD-PDT在体外能够抑制胆管癌QBC939细胞生长,这一效应主要是通过诱导QBC939细胞线粒体凋亡达到的,在凋亡过程中,出现了细胞色素C释放,caspase-9和-3的活化。结论 HPD-PDT体外能够抑制胆管癌细胞生长,诱导胆管癌细胞凋亡。更多还原

【Abstract】 Objective To investigate the effects of hematoporphyrin derivative-mediated photodynamic therapy (HPD-PDT) on cell growth in human cholangiocarcinoma in vitro and in vivo, as well as the underlying mechanisms of these effects. Methods 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay was used to evaluate growth status of human cholangiocarcinoma cell line(QBC939). Hoechst 33258 staining and flow cytometry assays were applied to determine cell apoptosis. Western blotting analysis was performed to detect the release of cytochrome c in QBC939 cells, and caspases enzymatic assay was used to investigate the activation of caspase-3,-8, and-9. Results HPD-PDT inhibits QBC939 cell growth via cell apoptosis in vitro, and initiates cell mitochondria apoptosis pathway by the release of cytochrome c and the activation of caspase-9 and-3. Conclusion HPD-PDT inhibits tumor growth and induces cell apoptosis of human cholangiocarcinoma, suggesting that HPD-PDT is useful in cholangiocarcinoma therapy.更多还原