【摘要】 目的探讨珊瑚羟基磷灰石(CHA)结合转血管内皮细胞生长因子(VEGF)基因的骨髓间充质干细胞构建的组织工程骨修复兔骨缺损的效果。方法 以CHA作为转VEGF基因骨髓间充质干细胞(BMSCs)的可吸附载体,体外构建组织工程骨,将其植入兔尺骨中段10mm骨缺损处,以CHA/BMSCs、自体骨移植组和空白组作为对照,在术后2、4、8、12周进行大体解剖、X线摄片、病理组织切片、生物力学测试,观察各组骨愈合程度及力学强度。结果 术后8周病理组织切片及X线摄片显示实验组骨缺损基本修复,效果优于对照组。生物力学测试显示术后12周组织工程骨组组抗扭转强度优于自体骨移植组。结论 CHA结合转VEGF基因BMSCs构建组织工程骨可用于骨缺损修复,优于自体骨移植组。更多还原

【Abstract】 Objective To investigate the efficacy of combined use of CHA and VEGF transfected BMSCs for repairing segmental bony defects.Methods CHA/VEGF/BMSCs composites were prepared and implanted in continuous defects in ulna of adult rabbits,while CHA/BMSCs,autograft implantation and no graft were used as controls.The animals were sacrificed on 2,4,8 and 12 weeks after operation.The bone repair process was investigated using physical,roentgenographical,vascularization,histological and biomechanical methods.Results Histological and roentgenographic examination showed that CHA/VEGF/BMSCs composite group had a more rapid repairing,compared with control groups after 8 weeks.Biomechanic tests showed that the antibending force on the CHA/VEGF/BMSCs side was stronger than it on the autograft implantation side after 12 weeks.Conclusions CHA/VEGF/BMSCs can efficiently repair bony defects,and is the best material.更多还原