【摘要】 目的:了解紫花地丁的抗菌活性及其活性成分。方法:化学成分的分离采用溶剂提取、硅胶柱色谱分离、Sephed-ex LH-20柱色谱和重结晶法进行,活性跟踪测定采用纸片扩散试验法进行,最小抑菌浓度采用比浊法进行,最小杀菌浓度采用比浊法并结合平皿培养来完成。结果:从紫花地丁全草的甲醇提取物中分离鉴定出4个具有抗菌活性的香豆素成分:七叶内酯(1),6,7-二甲氧基香豆素(2),东莨菪内酯(3)和5-甲氧基-7-羟甲基香豆素(4),其中4为新化合物。4个化合物对金黄色葡萄球菌、大肠杆菌、无乳链球菌、乳房链球菌、停乳链球菌和沙门氏菌均有不同程度的抑菌和杀菌活性,其中1的活性最好,对6种供试菌的MIC 0.031～0.313 g.L-1,MBC 0.313～0.625 g.L-1。结论:紫花地丁对动物病原菌具有较广谱的抗菌活性,香豆素是其主要的抗菌活性成分。更多还原

【Abstract】 Objective: Study on the antibacterial activity of Viola yedoensis and the antibacterial active compounds.Method: The chemical compositions were isolated by means of solvent extraction,column chromatography on silica gel,sephadex LH-20 and crystallization.The antibacterial activities were tested by Neo-Sensitab disk-diffusion method,nephelometric analysis and plating method.Result: One new compound(4) along with three known compounds were isolated from this plant for the first time and were identified as aesculetin(1),6,7-dimethoxycoumarin(2),scopoletin(3) and 5-methoxy-7-hydroxymethylcoumarin(4),respectively.All the compounds showed antibacterial and antibactericidal activities at varying degree on Streptococcus.Aureas,S.agalactiae,S.uberis,S.dysgalactiae,E.coli and Salmonella,of which 1 was most active with 0.031-0.313 g·L-1 of minimal inhibitory concentrations(MIC) and 0.313-0.625 g·L-1of minimal bactericidal concentrations(MBC).Conclusion: Viola yedoensis has a broad spectrum of antibacterial activity on animal pathogenic bacteria,and coumarins may be the main antibacterial activity ingredients.更多还原