【摘要】 目的:观察重组天花粉蛋白(recombinant trichosanthin,rTCS)高表达对人宫颈癌Caski细胞增殖的影响。方法:构建真核表达质粒pcDNA3.1(-)/6His-TCS,稳定转染Caski细胞后,RT-PCR,Western-blot筛选rTCS高表达细胞株。以转染空载质粒pcDNA3.1(-)细胞株作为对照组,MTT法检测2组细胞的增殖情况,流式细胞术检测细胞周期变化。结果:成功获得高表达rTCS的Caski细胞株,rTCS高表达能够显著抑制Caski细胞增殖(P<0.01),并能将细胞抑制在G1和G2期(P<0.05)。结论:rTCS在细胞内高表达具有抗人宫颈癌细胞生长的作用,该研究为宫颈癌治疗提供了新的思路。更多还原

【Abstract】 Objective: To observe the effects of high expression of recombinant trichosanthin(rTCS) on the cell proliferation and cell cycle of human cervical cancer Caski cells.Method: Eukaryotic expression plasmid pcDNA3.1(-)/6His-TCS was constracted and stably transfected into Caski cells.RT-PCR,Western-blot were used to select the clones with rTCS high-expressing.Using pcDNA3.1(-)-transfected cells as the control,MTT assay and flowcytometry were used to elucidate the effects of rTCS high expression on cell growth and cycle regulation in Caski cells.Result: The Caski cells with stable high expression of rTCS was successfully established,which could inhibit the cell growth(P<0.01) and arrest Caski cells in G1 and G2 phases(P<0.05) obviously.Conclusion: High expression of rTCS can inhibit the growth of Caski cervical cancer cells,which might provide a new pathway for the therapy of cervical cancer.更多还原