【摘要】 目的:建立一种用于测定壮药白花九里明中原儿茶酸、绿原酸和咖啡酸含量的HPLC方法,并探索不同采收月份药材中上述各成分的含量变化规律,为合理开发利用资源提供实验依据。方法:采用C18柱;以乙腈-0.1%磷酸溶液为流动相梯度洗脱,流速为1 mL·min-1,检测波长258,327 nm。结果:原儿茶酸在1.7~17 mg·L-1,绿原酸在15.6~156 mg·L-1,咖啡酸在3.963~39.63 mg·L-1具有良好的线性;平均加样回收率为101.5%,101.1%,99.26%。结论:所建立的方法简便、准确,可用于壮药白花九里明的质量评价。更多还原

【Abstract】 Objective: To develop an HPLC method for content determination of protocatechuic acid,chlorogenic acid and caffeic acid in Zhuang medicine Blumea megacephala(Randeria),and explore the content variation of the 3 components of the herbs harvesting in different months,and provide the scientific basis of reasonable application.Method: The determination was carried out on a Shimadzu VP-ODS column(4.6 mm×250 mm,5 μm) eluted with acetonitrile and water containing 0.1% phosphoric acid in a linear gradient elution.The flow rate was 1mL min-1,and the detected wavelength was set at 258,327nm.Result: The peak areas and the concentrations of the three components had good linear relationship in the range of 1.7-17 mg·L-1 for protocatechuic acid,15.6-156 mg·L-1 for chlorogenic acid,3.96-39.6 mg·L-1 for caffeic acid.The average recoveries were 103.4%,102.2%,98.5%,respectively.Conclusion: The method was proved to be simple,accurate and used for the quality evaluation of Blumea megacephala(Randeria).更多还原