【摘要】 目的:探讨芒果苷的抗炎作用机制。方法:脂多糖(LPS)+烟雾诱导法建立大鼠慢性支气管炎模型,测定肺泡灌洗液(BALF)和血清中的超氧化岐化酶(SOD)活性、丙二醛(MDA)、一氧化氮(NO)含量,肺组织中肿瘤坏死因子(TNF-α)、白细胞介素8(IL-8)含量;逆转录聚合酶链反应(RT-PCR)检测LPS诱导RAW264.7小鼠巨噬细胞环氧化酶-2(COX-2)mRNA的表达。结果:灌胃芒果苷高、中、低剂量(400,200,100 mg.kg-1)均使慢性支气管炎大鼠BALF和血清中的SOD活性、NO含量明显升高,MDA含量明显降低;肺组织中TNF-α,IL-8含量明显降低。200,100,50μmol.L-1芒果苷均可显著降低LPS诱导下的RAW264.7 COX-2 mRNA表达。结论:芒果苷抗炎机制可能与升高SOD活性,NO含量,降低MDA含量,下调TNF-α,IL-8,COX-2 mRNA的表达,减轻炎症反应有关。更多还原

【Abstract】 Objective: To explore the mechanism of anti-inflammatory effect of mangiferin.Method: The model of chronic bronchitis in rat was established by LPS+smoke.The activity of SOD,content of MDA and NO in BALF and serum,content of TNF-α and IL-8 were determined.The expression of RAW264.7 macrophage COX-2 mRNA induced by LPS in mice was detected by RT-PCR.Result: The activity of SOD,the content of NO in BALF and serum in rat with chronic bronchitis were significantly higher with high,medium and low-dose of lg mangiferin(400,200,100 mg·kg-1),while the content of MDA,and the content of TNF-α and IL-8 in lung tissues were lower.The expression of RAW264.7 macrophage COX-2 mRNA induced by LPS was significantly reduced by mangiferin with 200,100,50 μmol·L-1.Conclusion: The anti-inflammatory mechanism of mangiferin is to relieve inflammation by raising the activity of SOD and content of NO and reducing the content of MDA and the expression of TNF-α,IL-8 and COX-2 mRNA.更多还原