【摘要】 目的:观察全蝎蜈蚣对CIA大鼠外周血和小肠黏膜T细胞亚群的影响。方法:60只Wistar大鼠除正常组外均复制胶原免疫性关节炎后,并随机分成模型组、全蝎蜈蚣高、中、低剂量组和Ⅱ型胶原蛋白治疗组,给药40 d,采用流式细胞术检测大鼠外周血和小肠黏膜T细胞亚群水平。结果:与正常组比较,模型组大鼠外周血和PP结T淋巴细胞均表现为CD4+T细胞明显升高或有升高趋势,CD4+/CD8+则在外周血升高而在PP结下降(P<0.05或P<0.01)。与模型组比较,所有治疗组对CD3+T淋巴细胞作用不明显,全蝎蜈蚣各剂量组均可明显下调PP结CD4+,CD8+T细胞水平,升高CD4+/CD8+水平(P<0.05或P<0.01),同时外周血方面高、中剂量组CD4+T淋巴细胞水平及CD4+/CD8+显著下降(P<0.05或P<0.01),但对CD8+T淋巴细胞作用不明显。结论:全蝎蜈蚣治疗类风湿性关节炎的可能途径是调节CIA大鼠外周血和肠黏膜局部T淋巴细胞平衡。更多还原

【Abstract】 Objective:To observe effect of oral scorpio and scolopendra powder on T-cell subsets in peripheral blood and intestine from rats with collagen induced arthritis(CIA).Method:60 rats were randomly divided into 6 groups:normal control group,model control group,low-dose scorpio and scolopendra group,middle-dose scorpio and scolopendra group,high-dose scorpio and scolopendra group,and type Ⅱcollagen group.Rat′s rheumatoid arthritis was induced by collagen Ⅱ(CⅡ).Level of T-cell subsets from peripheral blood and intestine was measured by flow cytometry.Result:CD4+T cellular level was obviously increased(P<0.05 or P<0.01) or kept increased tendency in peripheral blood and intestine from the model group compared with that of the normal group,while the ratio of CD4+/CD8+ in intestine was obviously descent but the contrary in peripheral blood(P<0.05 or P<0.01).CD4+,CD8+ T cellular level in intestine were obviously descent and the ratio of CD4+/CD8+ increased in all treated groups when compared with in the model group(P<0.05 or P<0.01).However,CD4+ T cellular level and the ratio of CD4+/CD8+ in peripheral blood were remarkablely decreased.Conclusion:The mechanism that scorpio and scolopendra could treat rat′s rheumatoid arthritis may be regulating balance of T-lymphocyte subsets in peripheral blood and intestine.更多还原