【摘要】 目的:利用基因芯片技术分析肝癌HepG2细胞和正常肝上皮LO2细胞中miRNA的表达,并对HepG2细胞中低表达的miRNA-122a进行靶基因预测及相关生物信息学分析,为以miRNA-122a为靶点的基因治疗提供理论和实验基础。方法:利用基因芯片技术检测HepG2细胞和LO2细胞中miRNA-122a表达水平,通过生物信息学预测miRNA-122a的靶基因,并对其靶基因进行功能富集分析(GO-analysis)、信号转导通路富集分析(Pathway-analysis)和蛋白质相互作用网络分析。结果:与LO2细胞比较,miRNA-122a在HepG2细胞中呈低表达。miRNA-122a预测靶基因有1104个,其靶基因集合功能分别富集于碳水化合物生物合成、核苷酸代谢、细胞因子受体结合、细胞周期等生物学过程(P<0.001);信号转导通路显著富集于JAK-STAT信号通路、Wnt信号通路、MAPK信号通路、ErbB信号通路、细胞周期等信号转导通路(P<0.001)。结论:miRNA-122a在HepG2细胞中呈现低表达,miRNA-122a预测靶基因集合显著富集在与肿瘤发生相关的信号通路中。更多还原

【Abstract】 Objective:The present study aimed to investigate miRNA expression patterns in hepatocellular carcinoma(HepG2) and normol liver epithelial(LO2) cell lines.Another aim was to bioinformatically analyze as well as predict the target genes of miR-122a to provide both theoretical and experimental basis for gene therapy.Methods:The expression levels of miRNA-122a in HepG2 and L02 cells were detected using the gene chip technology.The bioinformatic analysis of the target genes of miRNA-122a involved enrichment(gene ontology),signal transduction pathway enrichment,and protein interaction network analyses.Results:miRNA -122a expression significantly decreased in HepG2 cells,compared with LO2 cells.The number of miRNA-122a target genes was 1104.The functions of these target genes were enriched in carbohydrate biosynthesis,nucleotide metabolism,cytokine receptor binding, cell cycle,and other biological processes(P<0.001).The JAK-STAT signaling,Wnt signaling,MAPK signaling,ErbB signaling, and cell cycle signal transduction pathways were significantly enriched(P<0.001).Conclusion:miRNA-122a expression significantly decreased in HepG2 cells.Some of the predicted target genes of miRNA-122a were significantly enriched in tumor related with signaling pathways.更多还原