【摘要】 目的:探讨茅莓总皂苷对缺血性再灌注后凋亡相关基因Bcl-2mRNA,BaxmRNA表达的影响。方法:采用大鼠大脑中动脉阻塞造成局灶性脑缺血再灌注模型,以原位杂交技术观察脑缺血再灌注不同时间点茅莓总皂苷5,10,20 mg.kg-1对凋亡相关基因Bcl-2 mRNA,Bax mRNA的表达。结果:3个剂量的茅莓总皂苷治疗组增加Bcl-2 mRNA阳性细胞的表达,降低Bax mRNA阳性细胞的表达。结论:茅莓总皂苷有明显的抗凋亡作用可能与增加Bcl-2 mRNA阳性细胞的表达,降低BaxmRNA阳性细胞的表达有关。更多还原

【Abstract】 OBJECTIVE To explore the effects of TSRP on the expression of Bcl-2 mRNA,Bax mRNA genes after focal cerebral ischemic reperfusion in rats. METHODS The middle cerebral artery was occluded for 2h to result in the focal ischemic.followed by reperfusion.The expression of Bcl-2 mRNA,Bax mRNA was determined by in situ hybridization. RESULTS The expression of Bax mRNA positive cells in the TSRP groups significantly decreased as compare with the control group;however,the expression of Bcl-2mRNA positive cells increased significantly as compare with the control group. CONCLUSION The anti-apoptotis mechanism of TSRP may contribute to the increased expression of Bcl-2 mRNA,at the same time,decreased expression of Bax mRNA.更多还原