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茅莓总皂苷对大鼠局灶性脑缺血/再灌注后凋亡相关基因Bcl-2mRNA,BaxmRNA表达的影响

Effects of total saponins of Rubus parviflolius L(TSRP) on neuronal apoptotic related gene Bcl-2,Bax in rats after local cerebral ischemic-reperfusion

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【作者】 王继生邱宗荫夏永鹏李惠芝

【Author】 WANG Ji-sheng1,QIU Zong-yin2,XIA Yong-peng2,LI Hui-zhi2 (1.The Third Hospital of Mianyang,Sichuan Mianyang 621000,China;2.Department of pharmacy,Chongqing University of Medical Science,Chongqing 400016,China)

【机构】 绵阳市第三人民医院重庆医科大学药学院

【摘要】 目的:探讨茅莓总皂苷对缺血性再灌注后凋亡相关基因Bcl-2mRNA,BaxmRNA表达的影响。方法:采用大鼠大脑中动脉阻塞造成局灶性脑缺血再灌注模型,以原位杂交技术观察脑缺血再灌注不同时间点茅莓总皂苷5,10,20 mg.kg-1对凋亡相关基因Bcl-2 mRNA,Bax mRNA的表达。结果:3个剂量的茅莓总皂苷治疗组增加Bcl-2 mRNA阳性细胞的表达,降低Bax mRNA阳性细胞的表达。结论:茅莓总皂苷有明显的抗凋亡作用可能与增加Bcl-2 mRNA阳性细胞的表达,降低BaxmRNA阳性细胞的表达有关。

【Abstract】 OBJECTIVE To explore the effects of TSRP on the expression of Bcl-2 mRNA,Bax mRNA genes after focal cerebral ischemic reperfusion in rats. METHODS The middle cerebral artery was occluded for 2h to result in the focal ischemic.followed by reperfusion.The expression of Bcl-2 mRNA,Bax mRNA was determined by in situ hybridization. RESULTS The expression of Bax mRNA positive cells in the TSRP groups significantly decreased as compare with the control group;however,the expression of Bcl-2mRNA positive cells increased significantly as compare with the control group. CONCLUSION The anti-apoptotis mechanism of TSRP may contribute to the increased expression of Bcl-2 mRNA,at the same time,decreased expression of Bax mRNA.

【基金】 重庆市重大科技专项资助课题[编号:渝科发计字(2004)27号]
  • 【文献出处】 中国医院药学杂志 ,Chinese Journal of Hospital Pharmacy , 编辑部邮箱 ,2011年18期
  • 【分类号】R285.5
  • 【被引频次】5
  • 【下载频次】121
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