【摘要】 目的:研究板栗毛壳水提液(AEICM)对大鼠全脑缺血再灌注损伤的保护作用。方法:采用板栗毛壳水提液(AE-ICM)对大鼠连续灌胃7d后,利用4-血管阻断(4-VO)法建立全脑缺血再灌注动物模型,采用干湿质量法观察板栗毛壳水提液(AEICM)对脑水肿的影响,利用分光光度法检测大鼠脑、肝、肾、心等组织中丙二醛(MDA)水平、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-PX)活性。结果:AEICM能显著减轻脑水肿(P<0.05),降低MDA(P<0.01)的水平,增加各器官组织中SOD(P<0.01或P<0.05)和(GSH-PX)(P<0.05)活性。结论:AEICM对脑缺血再灌注损伤有保护作用,其机制可能与抗脂质过氧化有关。更多还原

【Abstract】 OBJECTIVE To study the protective effects of aqueous extract of Involucrum Castaneae Mollissimae(AEICM) on the whole cerebral ischemia-reperfusion injury in rats.METHODS Thirty mice were randomly divided into the normal group,model group and AEICM group.AEICM was given to the AEICM group orally.After 7 days,the whole cerebral ischemia reperfusion injury was established by four-vessel occlusion(4-VO).Dry-wet weight method was carried out to study the protective effect of AEICM on brain edema in rats.The activities of SOD and GSH-PX and the level of MDA in the brain,heart,liver,kidney tissues were determined by the ultraviolet spectrophotometer.RESULTS Compared with the model group,the activities of SOD and GSH-PX(P<0.01,P<0.05) were enhanced remarkably by AEICM in all tissues,but the water content of brain tissue and the level of MDA were decreased remarkably(P<0.05).CONCLUSION AEICM had protective effects on the whole cerebral ischemia reperfusion injury in rats,and the mechanism may relate to its inhibitory effect on the lipid peroxidation.更多还原