【摘要】 目的:观察吉西他滨在体外对人胚肾细胞HEK293和人乳腺癌细胞MCF-7增殖的影响。方法:采用MTT法测定吉西他滨对人胚肾细胞HEK293和人乳腺癌细胞MCF-7增殖的影响,比较两者IC50值,绘制细胞生长曲线;并测定吉西他滨作用后细胞培养上清液中SOD,MDA,LDH值的变化。结果:吉西他滨在体外对人乳腺癌细胞MCF-7的增殖抑制作用较强,同时对人胚肾细胞HEK293的增殖有一定的抑制作用,两者的化疗指数为分别为26.907(24h),15.307(48h),10.264(72h)。结论:吉西他滨对人乳腺癌细胞MCF-7有明显增殖抑制作用,同时可抑制人胚肾细胞HEK293的增殖,对肾脏有一定的毒性。更多还原

【Abstract】 OBJECTIVE To demonstrate the antiproliferative effect of gemcitabine on human ermbryonic kidney cell 293 and human breast cancer cell MCF-7 in vitro.METHODS The inhibiting rate of gemcitabine on human ermbryonic kidney cell 293 and human breast cancer cell MCF-7 was detected by MTT assay.Their IC50 values for the effect of gemcitabine were compared.The cell growth curve was designed.The contents of SOD,MDA and LDH in cultural supernate were measured by ultraviolet spectrophotometry.RESULTS Gemcitabine significantly inhibited the proliferation of human breast cancer cell MCF-7 in vitro.It also inhibited the proliferation of human ermbryonic kidney cell 293.Their chemotherapeutic index were 26.907(24 h),15.307(48 h) and 10.264(72 h).CONCLUSION Gemcitabine is cytotoxic in human breast cancer cell MCF-7,and inhibits the proliferation of human ermbryonic kidney cell 293.更多还原