【摘要】 目的采用HPLC测定咪达唑仑在小鼠体内的分布情况。方法采用Kromasil C18色谱柱(4.6 mm×150 mm,5μm),甲醇-水(含0.1%二乙胺,0.05%磷酸溶液,60∶40)为流动相,流速1.0 mL.min-1,检测波长225 nm。以三唑仑为内标物,按内标法定量。结果咪达唑仑在5～400 ng.mL-1内线性关系良好(r=0.999 3),咪达唑仑最低检测质量浓度为2.5 ng.mL-1,平均回收率为(96.95±1.8)%,日内及日间的RSD均小于2%。结论本方法具有快速、简便、灵敏、准确、经济等特点,适用于测定咪达唑仑在小鼠体内的分布,为该药物的临床检测提供依据。更多还原

【Abstract】 OBJECTIVE Determination by HPLC of midazolam in mouse distribution,and the method was applied to detect midazolam in mouse distribution.METHODS The analytical column was packed with Kromasil C18(4.6 mm×150 mm,5 μm) the mobile phase was methanol-water 60∶40,containing 0.1% diethyl amine,0.05%phosphoric acid,the flow rate was 1 mL·min-1,and detection wave length was set at 225 nm,with the triazolam as the internal standard,according to the internal standard to quantitate.RESULTS The linear ranges were 5-400 ng·mL-1 for midazolam in human plasma.The correlation coefficients were 0.999 3.The midazolam low limit of detection was 2.5 ng·mL-1.The method recoveries were(96.95±1.8)%.Withinday and betweenday RSD were less than 2%.CONCLUSION The method is rapid,simple,sensitive,accurate and economic,and applied to provide basis for clinical testing for midazolam in mouse distribution.更多还原