【摘要】 利用模式植物拟南芥AtPex16p/SSE1基因序列与油菜数据库BBSRC BrassicaDB比对,得到甘蓝型油菜(Brassica napus)同源EST序列,拼接出油菜Pex16p基因全长cDNA电子克隆,然后设计全长引物,以甘蓝型油菜种子cDNA为模板,克隆Pex16p基因,得到全长为1 101bp的cDNA,编码366个氨基酸的蛋白,命名为BnPex16p。Bn-Pex16p基因序列与拟南芥AtPex16p/SSE1同源,其蛋白与拟南芥同源蛋白具有完全相同的PTS2型过氧化物酶体定位肽,进化关系相近。半定量PCR(RT-PCR)发现BnPex16p基因在油菜幼嫩的根、茎、叶和种子中均有高丰度表达,在种子发育过程的中期和后期表达水平增加,暗示该基因在油脂的积累中有重要作用。更多还原

【Abstract】 According to AtPex16p/SSE1 and ESTs sequences from Brassica napus by wu-blast searching in BBSRC BrassicaDBA,a full-length cDNA encoding Pex16p from developing seeds of Brassica napus var CY2 was cloned.The gene was designated as BnPex16p which was 1 101bp in length encoding 366 amino acids.It was found that BnPex16p gene was homologous to AtPex16p/SSE1.They both had a PTS2 type peroxisome targeting signal and a close evolutionary distance.Semi-quantitative RT-PCR analysis demonstrated that BnPex16p was constitutively expressed in CY2 tissues and its expression increased in seeds during the developmental stages.It indicated that BnPex16p gene might have crucial roles in oil accumulation in B.napus.更多还原