【摘要】 目的观察黄绿青霉素(CIT)对血管内皮细胞凋亡和DNA损伤的作用。方法体外原代培养人脐静脉内皮细胞,给予浓度分别为1μmol/L、2μmol/L、5μmol/L、10μmol/L的CIT毒素,处理时间为48h。光镜观察细胞生长形态,MMT法检测细胞活性,流式细胞仪检测细胞凋亡情况,单细胞凝胶电泳法分析CIT对内皮细胞DNA损伤作用。结果对照组细胞生长良好,呈梭形,CIT组生长状态较差,部分细胞呈圆形,有坏死;2μmol/L、5μmol/L和10μmol/L CIT染毒组细胞活性低于对照组(P<0.05);2μmol/L、5μmol/L和10μmol/L染毒组细胞凋亡率高于对照组(P<0.05);5μmol/L和10μmol/L染毒组DNA损伤较对照组严重(P<0.05)。结论 CIT可降低人脐静脉血管内皮细胞活性,诱导细胞凋亡,引发DNA损伤。更多还原

【Abstract】 Objective To investigate the effects of apoptosis and DNA damage on human umbilical vein endothelial cells(HUVEC) caused by citreoviridin.Methods HUVECs were isolated from the neonatal umbilical core in 1 hour(with informed consent form of the family) and cultured.The injury models were established by dealing with CIT at different concentrations of 1 μmol/L,2 μmol/L,5 μmol/L and 10 μmol/L for 48 hours.Cells′ morphous was observed under light microscope during the period of growth.Levels of cells′ viability were assessed by MTT assay.Flow cytometry was used to demonstrate apoptotic changes.Single cell gel electrophoresis(SCGE) was used to measure the DNA damage of the cells.Results Cells in control grew better than CIT groups,Some of the cells in CIT groups were round.The level of cells′ viability in control was higher than those of CIT groups at doses of 2 μmol/L,5 μmol/L and 10μmol/L(P<0.05).Apoptotic rates in CIT groups of 2 μmol/L,5 μmol/L and 10 μmol/L were higher than that of the control.The levels of damaged DNA in CIT groups of 5 μmol/L and 10 μmol/L were significantly diffenent from that of the control.Conclusion CIT causes down-regulation of the viability of HUVEC,induces apoptosis of HUVEC and results in DNA damage.更多还原