【摘要】 目的:探讨维生素A(VA)对稳定期慢性阻塞性肺疾病(COPD)患者细胞因子表达的影响。方法:将84例稳定期COPD患者随机均分为对照组、治疗1组及治疗2组,2个治疗组分别接受为期6个月的口服VA治疗,剂量分别为5000、10000u。采用高效液相色谱法测定患者血清中VA的浓度,放射免疫法测定治疗前、后患者诱导痰及血清细胞因子白细胞介素(IL)-8、IL-1β、肿瘤坏死因子(TNF-α)的水平,并记录不良反应。结果:治疗后3组患者血清VA浓度有所上升,具有时间和剂量依赖性,与治疗前比较差异有统计学意义(P<0.05)。补充VA1个月后,3组细胞因子水平较治疗前无明显增加,组间比较无统计学差异(P>0.05);3个月后,2个治疗组细胞因子水平较治疗前有所下降(P<0.05),但3组间比较无统计学差异(P>0.05);6个月后,2个治疗组细胞因子水平较3个月时进一步下降,3组比较差异有统计学意义(P<0.05),2个治疗组细胞因子水平均明显低于对照组(P<0.05),且治疗2组明显低于治疗1组(P<0.05)。结论:VA可以下调细胞因子IL-8、IL-1β、TNF-α的表达,进而抑制稳定期COPD患者的系统炎症反应。更多还原

【Abstract】 OBJECTIVE: To investigate the effect of vitamin A (VA) on cytokines expression of patients with chronic obstructive pulmonary disease (COPD) in stable stage. METHODS: 84 COPD patients in stable stage were randomly divided into 3 groups: first treatment group (VA 5 000 u), second treatment group (VA 10 000 u) and control group. They were given oral dose of VA for 6 months. Adverse drug reactions were recorded. The serum concentration of VA was determined by HPLC. The levels of IL-8, IL-1β and TNF-α in serum and induced sputum were measured with RIA. RESULTS: The concentration of vitamin A was increased after treatment in time and dose dependent manners in 3 groups, there was statistical significance(P<0.05). After 1 month of treatment, cytokines levels had no significant change, there were statistical significance(P>0.05). The cytokines levels were lowered after 3 month(P<0.05). There was no statistical difference among 3 groups(P>0.05). After 6 months, the cytokines levels were significantly decreased in treatment groups, compared with after 3 months, there was statistical significance. The cytokines levels of treatment groups were lower than those of control group (P<0.05). Those of second treatment group were lower than those of first treatment group(P<0.05). CONCLUSION: VA could down-regulate the levels of IL-8, IL-1β and TNF-α and may restrain systemic inflammatory reaction of COPD patients in stable stage.更多还原