【摘要】 目的:建立测定胆香鼻炎片中猪去氧胆酸含量的方法。方法:以1-溴乙酰基对硝基苯为衍生化试剂,18-冠醚-6为催化剂,采用反相高效液相色谱法分析。色谱柱为Diamonsil C18(250mm×4.6mm,5μm),流动相为乙腈-水(60:40),流速为1.0mL·min-1,检测波长为254nm。结果:猪去氧胆酸的检测浓度在5.255～105.100μg·mL-1范围内与峰面积积分值呈良好的线性关系(r=0.9994);平均加样回收率为97.64%,RSD=2.1%(n=6)。结论:本方法简便、准确、重复性好,可用于胆香鼻炎片中猪去氧胆酸的含量测定,并为该制剂的质量控制提供依据。更多还原

【Abstract】 OBJECTIVE:To determine the content of hyodeoxycholic acid in Danxiang biyan tablets.METHODS:After with 18-crown ether-6 as the catalyst and 1-(2-bromoacetyl)-p-nitophenylacetonitrile as the derivatization reagent,the determination was carried out on a Diamonsil C18 column by RP-HPLC with mobile phase consisted of acetonitrile-water (60 ∶ 40) at the flow rate of 1.0 mL·min-1.The detection wavelength was set at 254 nm.RESULTS:The linear range of hyodeoxycholic acid was 5.255～105.100 μg·mL-1(r=0.999 4) with an average recovery of 97.64%(RSD=2.1%,n=6).CONCLUSION:The method is simple,accurate and reproducible for the content determination of hyodeoxycholic and the quality control of Danxiang biyan tablets.更多还原