【摘要】 目的:建立测定木薯叶中芦丁含量的方法。方法:采用高效液相色谱法。色谱柱为Agilent ZORBAX Eclipse XDB C18(0.105006～mm0.×046.06mmgm.,m5Lμ-1m范)围,流内动与相峰为面甲积醇积-分0.1值%呈磷良酸好(1的2:线88性),关流系速(为r=10.m99L.9m9)i;n-平1,均检加测样波回长收为率35为4n1m00。.7结2%果,:R芦SD丁=的2.检3%测(浓n=度6)在。结论:本方法操作简便、准确、重复性好,可作为木薯叶中芦丁的含量测定方法。更多还原

【Abstract】 OBJECTIVE:To establish a method for the content determination of rutin in Manihot esculenta leaves.METHODS:HPLC method was adopted.Agilent ZORBAX Eclipse XDB C18(150 mm×4.6 mm,5 μm) column was used with mobile phase consisted of methanol-0.1% phosphoric acid(12:88) at flow rate of 1 mL·min-1.The detection wavelength was set at 354 nm.RESULTS:The linear range of rutin was 0.006～0.060 mg·mL-1(r=0.999 9) with an average recovery of 100.72%(RSD=2.3%,n=6).CONCLUSION:The method is simple,accurate,reproducible and could be used for the content determination of rutin in M.esculenta leaves.更多还原