【摘要】 目的:建立异甘草素固体脂质纳米粒(ISO-SLN)包封率测定方法。方法:采用透析法分离样品中的游离药物及载药固体脂质纳米粒,并确定透析介质和透析时间;紫外分光光度法测定包封率,检测波长为396nm。结果:以2%泊洛沙姆188水溶液为透析介质、透析时间为8h,能有效分离ISO-SLN与游离ISO,加样回收率大于95%;ISO检测浓度线性范围为1.12～7.84μg·mL-1(r=0.9995),低、中、高浓度平均回收率为99.54%、99.11%、100.2%,RSD均小于5%;3批ISO-SLN平均包封率为80.2%。结论:所建立的方法可用于测定ISO-SLN包封率,且方法准确、简便。更多还原

【Abstract】 OBJECTIVE:To establish the method for encapsulation efficiency determination of Isoliquiritigenin solid lipid nanoparticles(ISO-SLN) .METHODS:Dialysis method was used to isolate free fraction and drug-loading solid lipid nanoparticles from samples.Medium and time of dialysis were confirmed.The encapsulation efficiency was determined by UV spectrophotometry at detection wavelength of 396 nm.RESULTS:2% poloxamer188 hydrate medium was used as dialysis medium and dialysis time was 8 h.Free ISO could be effectively separated from ISO-SLN by dialysis method.The recovery rate was more than 95%.The linear range of ISO was 1.12～7.84 μg·mL-1(r=0.999 5) with average recoveries of 99.54%,99.11% and 100.2% at low,medium and high concentrations(RSD<5%) .The encapsulation efficiency of 3 batches of ISO-SLN was 80.2% averagely.CONCLUSION:The method is accurate and simple for encapsulation efficiency determination of ISO-SLN.更多还原