【摘要】 目的:建立同时测定大鼠血浆中紫杉醇、汉防己甲素的方法。方法:采用反相高效液相色谱法。取血浆200μL,以利血平为内标,利用乙醚提取,氮气吹干,残渣用流动相溶解,进样20μL。色谱柱为Platisisl ODS(250mm×4.6mm,5μm),流动相为甲醇-乙腈-0.01mo·lL-1K2HPO4(30∶40∶30,磷酸调节pH值至4.5),流速为1.0mL·min-1,检测波长为227nm。结果:紫杉醇、汉防己甲素检测浓度分别在0.02～20μg·mL-1和0.02～5μg·mL-1(r分别为0.9990、0.9993)范围内与各自峰面积积分值呈良好线性关系。紫杉醇和汉防己甲素低、中、高浓度的方法回收率分别为100.5%～104.0%和101.0%～104.8%,提取回收率分别为84.7%～93.8%和70.8%～86.0%;日内、日间精密度均<10%。结论:本方法专属性强、操作简单、结果准确,可用于紫杉醇和汉防己甲素在大鼠体内的药动学研究。更多还原

【Abstract】 OBJECTIVE:To establish the RP-HPLC method for the simultaneous determination of paclitaxel and tetrandrine in rats plasma. METHODS:With crystoserpine as the internal standard,the plasma sample 200 μL was extracted with ether,evaporated under gentle N2 stream and the residual was resolved with mobile phase. The samples were separated on Platisil ODS(250 mm×4.6 mm,5 μm) column with a mobile phase of methol-acetonitrile-0.01 mol·L-1 dipotassium hydrogen phosphate(30 ∶ 40 ∶ 30,pH adjusted to 4.5 with phosphoric acid) at the flow rate of 1.0 mL·min-1. The detection wavelength was set at 227 nm. RESULTS:The linear ranges were 0.02～20 μg·mL-1(r=0.999 0) for paclitaxel and 0.02～5 μg·mL-1(r=0.999 3) for tetrandrine. The method recovery was 100.5%～104.0% and 101.0%～104.8% and the extraction recovery was 84.7%～93.8% and 70.8%～86.0%,respectively. The RSD of intra-day and inter-day both were less than 10%. CONCLUSION:The method is specific,simple,accurate and reproducible for pharmacokinetics study of paclitaxel and tetrandrine in rats plasma.更多还原