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知母皂苷A-Ⅲ、知母皂苷C对照品的制备与鉴定
Preparation and Identification of Timosaponin A-Ⅲ and Timosaponin C
【摘要】 目的:研究知母中知母皂苷A-Ⅲ、知母皂苷C对照品的制备方法,并对其进行鉴定。方法:采用大孔吸附树脂、硅胶色谱、Sephadex LH-20色谱、反相ODS色谱分离制备对照品,采用红外、质谱、核磁等波谱方法进行结构鉴定。结果:从知母中分离得到知母皂苷A-Ⅲ与知母皂苷C对照品,纯度均>98%。结论:本试验结果可为知母皂苷的新药开发提供物质基础。
【Abstract】 OBJECTIVE:To investigate the preparation methods and identification of timosaponinA-Ⅲ and timosaponin C of Anemarrhena asphodeloides.METHODS:Substance control were isolated and prepared by macroporous adsorption resin,silica gel column and Sephadcx LH-20 column,reverse phase ODS chromatography column.The structures of timosaponinA-Ⅲ and timosaponin C of A.asphodeloides were identified by IR,MS,NMR spectrum.RESULTS:The purity of isolated timosaponin A-Ⅲ and timosaponin C from A.asphodeloides was more than 98%.CONCLUSION:Results of this study provide material basis for new drug development of timosaponin.
【Key words】 Anemarrhena asphodeloides; Timosaponin A-Ⅲ; Timosaponin C; Substance control; Preparation; Identification;
- 【文献出处】 中国药房 ,China Pharmacy , 编辑部邮箱 ,2011年15期
- 【分类号】R285
- 【下载频次】451