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知母皂苷A-Ⅲ、知母皂苷C对照品的制备与鉴定

Preparation and Identification of Timosaponin A-Ⅲ and Timosaponin C

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【作者】 欧阳辉黄小方周茂福涂世程徐伟亚杨世林

【Author】 OUYANG Hui,HUANG Xiao-fang,ZHOU Mao-fu,TU Shi-cheng,XU Wei-ya,YANG Shi-lin(Jiangxi University of Traditional Chinese Medicine,Nanchang 330025,China)

【机构】 江西中医学院

【摘要】 目的:研究知母中知母皂苷A-Ⅲ、知母皂苷C对照品的制备方法,并对其进行鉴定。方法:采用大孔吸附树脂、硅胶色谱、Sephadex LH-20色谱、反相ODS色谱分离制备对照品,采用红外、质谱、核磁等波谱方法进行结构鉴定。结果:从知母中分离得到知母皂苷A-Ⅲ与知母皂苷C对照品,纯度均>98%。结论:本试验结果可为知母皂苷的新药开发提供物质基础。

【Abstract】 OBJECTIVE:To investigate the preparation methods and identification of timosaponinA-Ⅲ and timosaponin C of Anemarrhena asphodeloides.METHODS:Substance control were isolated and prepared by macroporous adsorption resin,silica gel column and Sephadcx LH-20 column,reverse phase ODS chromatography column.The structures of timosaponinA-Ⅲ and timosaponin C of A.asphodeloides were identified by IR,MS,NMR spectrum.RESULTS:The purity of isolated timosaponin A-Ⅲ and timosaponin C from A.asphodeloides was more than 98%.CONCLUSION:Results of this study provide material basis for new drug development of timosaponin.

【基金】 科技部科技型中小企业技术创新基金资助项目(04C2621360123400)
  • 【分类号】R285
  • 【下载频次】451
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