【摘要】 目的:建立滋肾通关胶囊的高效液相色谱(HPLC)指纹图谱,用于评价其提取工艺的优劣。方法:色谱柱为Kromasil C18(250mm×4.6mm,5μm),流动相为乙腈-33mmol·L-1磷酸二氢钾缓冲液(梯度洗脱),检测波长为317nm,柱温为25℃,流速为1mL·min-1,进样量为10μL。用盐酸小檗碱、芒果苷、桂皮醛3种对照品为参照物,建立混合药材的指纹图谱,并以此为标准图谱指认了供试品中对应的成分。结果:在建立的色谱条件下,样品中的成分得到了较好的分离。以70%的乙醇为提取溶剂优于其他2种溶剂。结论:在滋肾通关胶囊的提取工艺中,指纹图谱的多成分评价方法优于常规的单一指标评价方法。更多还原

【Abstract】 OBJECTIVE:To establish the HPLC fingerprint of Zishen tongguan capsule and evaluate the pros and cons of extraction process.METHODS:The analytical column was Kromasil C18(250mm×4.6mm,5μm) column with acetonitrile-33mmol·L-1 monopotassium phosphate buffer solution (gradient elution) as mobile phase at flow rate of 1mL·min-1.The detection wavelength was set at 317nm and the column temperature was 25℃.The injection volume was 10μL using berberine hydrochloride,mangiferin and cinnamaldehyde as control.The HPLC fingerprint of mixed medicine was established and uses as standard chromatograms.The corresponding composition of samples had been identified.RESULTS:Chemical compositions of samples had been separated successfully by established method.70% ethanol was better than other two solvent for extracting.CONCLUSION:The multi-component evaluation method of HPLC fingerprint is better than the normal single-component evaluation method in the study of extraction process of Zishen tongguan capsule.更多还原