【摘要】 为了解广州地区犬流感的流行情况,本研究采集107份犬鼻咽拭子样品和58份血清样品,SPF鸡胚分离病毒并进行抗体检测。结果显示:分离获得3株H3N2亚型犬流感病毒。分离株的EID50为10-2.42/0.1 mL～10-3.5/0.1 mL;MDT为177.6 h～192 h;对乙醚、氯仿、酸和温度均敏感;对血凝素为热不稳定型;能够凝集公鸡、豚鼠、猪和牛的红细胞。更多还原

【Abstract】 To evaluate the prevalence of canine influenza virus(CIV) in Guangzhou area,samples of 107 nasopharynx swabs and 58 serum were collected from dogs for virus isolation and antibody detection.Three H3N2 subtype CIV were isolated.The 50% embryo infectious dose(EID50) of the three isolates were 10-2.42/0.1 mL to 10-3.5/0.1 mL respectively.The mean death time(MDT) ranged from 177.6 h to 192 h.All three isolates were sensitive to ether,chloroform acidum and heat,and the isolates possessed HA activity to erythrocytes of rooster,guineapigs,pig and cattle.更多还原