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水提—醇沉法提取佛手粗多糖的最佳工艺研究
Optimum Technique Research on the Extraction of Bergamot Crude Polysaccharide with Ethanol Subsiding Method
【摘要】 目的:确定利用传统方法提取佛手粗多糖的最佳工艺条件。方法:以佛手多糖的含量为指标,以提取时间、提取次数、提取温度、料液比为提取因素,通过单因素试验确定因素水平,利用统计分析方法对提取佛手粗多糖的工艺进行优化研究。结论:水提—醇沉法提取佛手粗多糖的最佳工艺条件是:提取温度95℃,浸提时间2h,料液比1:15,提取次数2次,此方法可以为工业大规模生产佛手多糖提供依据。
【Abstract】 【Objective】To determine the optimum condition of total sugar content of crude polysaccharide from bergamot.【Method】 Using polysaccharide content of bergamot as indicators,the extraction time,extraction times,extraction temperature,solid to liquid ratio as extraction factors,factor level were determined through single factor experiment,and the extraction optimization of bergamot crude polysaccharide was researched by statistical method.【Conclusion】 The optimum conditions of ethanol subsiding method to extract total sugar content of crude polysaccharides from bergamot were extraction temperature 95℃,extraction time 2h,solid to liquid ratio 1:15,and extraction twice.This method will provide basis for the mass industrial production of bergamot polysaccharide.
【Key words】 bergamot crude polysaccharide; traditional method; extraction technique; orthogonal test; statistical analysis;
- 【文献出处】 中国食物与营养 ,Food and Nutrition in China , 编辑部邮箱 ,2011年11期
- 【分类号】TQ461
- 【被引频次】9
- 【下载频次】575