【摘要】 目的:对祁州漏芦愈伤组织诱导培养基和生长的培养条件进行研究,构建祁州漏芦快速繁殖体系。方法:通过筛选外植体,添加不同种类及浓度的生长调节剂对祁州漏芦进行愈伤组织诱导、芽增殖培养及生根培养。结果:诱导愈伤组织适宜培养基:MS+0.3mg/L NAA+3.0mg/L 6-BA;芽增殖培养以MS+0.3mg/L NAA+5.0mg/L 6-BA为宜;生根培养以1/2MS+0.1mg/L NAA为佳。结论:利用组织培养技术能够实现祁州漏芦快速繁殖。更多还原

【Abstract】 【Objective】 To provide some new evidences for rapid propagation of Rhaponticum? uniflorum through studying on medium with callus tissue culture and the condition of growth.【Method】 Callus tissue induction,bug proliferation culture,rooting culture of Rhaponticum uniflorum were conducted by explants selecting and growth regulator adding different hormones.【Result】 The appropriate medium for different culture stages were MS+0.3mg/L NAA+3.0mg/L 6-BA in callus culture induction,MS+0.3mg/L NAA+5.0mg/L 6-BA in differentiation and subculture of cluster buds,1/2MS+0.1mg/L NAA in rooting culture.【Conclusion】Rapid propagation can be achieved with plant tissue culture in Rhaponticum uniflorum.更多还原