【摘要】 分别从新疆玛纳斯、乌兰巴依、南山、水西沟采集的4份酸驼乳中,分离得到乳酸菌22株。采用传统形态学、生理生化特性方法对乳酸菌进行鉴定,鉴定结果为Lactobacillus16株(72.72%),Lactococcus 3株(13.63%),Enterococcus 2株(9.10%),Streptococcus 1株(4.55%),优势杆菌为Lactobacillus helveticus(18.18%),优势球菌为Lactococcus lactis subsp.lactis(13.63%)。利用16S rDNA序列同源性分析和系统发育树分析对MLS1进行了分子鉴定,鉴定结果为菌株MLS1与Lactobacillus rhamnosus的同源性达到98.1%,与传统生理生化鉴定结果一致,表明了16S rDNA序列分析应用在乳酸菌鉴定上的准确性。更多还原

【Abstract】 Totally 22 strains of lactic acid bacteria(LAB)were isolated from horgormag sample which was gathered from Manasi,Wulanbayi,Nashan and Shuixigou of Xinjiang.According to phenotypic,physiological and biochemical properties strains isolated were identified as 16 strains of Lactobacillus(72.72%),3 strains of Lactococcus(13.63%),2 strains of Enterococcus(9.10%),I strain of Streptococcus(4.55%).The dominated species found were Lactobacillus helveticus(18.18%)and Lactococcus lactis subsp.Lactis(13.63%).Strain of MlS1 was indentified by molecular method of 16S rDNA homology analysis and phylogenetic tree analysis.The homology of MlS1 and Lactobacillus rhamnosus was 98.1%.It was coincident with indentification of physiological and biochemica to show that the identification by sequence analysis of 16S rDNA on lactic acid bacteria was accurate.更多还原