【摘要】 目的检测幽门螺杆菌(Helicobacter pylori,H.pylori)感染阳性的胃部疾病患者外周血中CD4+CD25+调节性T细胞(Treg细胞)的百分含量及转化生长因子-β1(transforming growth factor-β1,TGF-β1)的水平,探讨CD4+CD25+调节性T细胞在H.pylori感染中的免疫调节作用及意义。方法采用流式细胞术检测H.pylori感染的慢性浅表性胃炎、胃癌前病变和胃癌患者外周血中CD4+CD25+调节性T细胞的含量、CD4+CD25+T细胞中表达FOXP3的细胞比例;并采用ELISA方法检测H.pylori感染者血清中TGF-β1的含量,无H.pylori感染的患者作为阴性对照。结果 H.pylori感染的患者外周血中CD4+CD25+调节性T细胞的百分含量及TGF-β1的水平较不伴有H.pylori感染的患者显著升高(P<0.05);H.pylori感染的浅表性胃炎、胃癌前病变及胃癌患者外周血中CD4+CD25+T淋巴细胞的百分含量及CD4+CD25+T细胞中表达FOXP3的细胞比例随病变严重程度的进展逐渐升高,差异有统计学意义(P<0.05);H.pylori感染的患者血清中TGF-β1水平也随病变严重程度的进展逐渐升高,差异有统计学意义(P<0.05)。结论 H.pylori感染可增加CD4+CD25+调节性T细胞的含量和TGF-β1的水平;随着病变严重程度的进展,CD4+CD25+调节性T细胞的含量和TGF-β1的水平逐渐升高,CD4+CD25+调节性T细胞百分含量和TGF-β1水平可作为临床判断病情进展的指标。更多还原

【Abstract】 Objective To detect the level of CD4+CD25+ T cells and TGF-β1 in Helicobacter pylori(H.pylori)-associated gastric disease patients,and investigate the role and significance of CD4+CD25+ T cells in immune regulation of HP infection.Method The percentage of CD4+CD25+ T cells and CD4+CD25+FOXP3+ subsets of patients with H.pylori infection were detected by flow cytometry,and enzyme linked immunosorbent assay(ELISA) was employed to determine the levels of serum TGF-β1.Cases included chronic superficial gastritis(CSG),preneoplastic gastric lesions(PGL) and gastric cancer(GC).Cases without H.pylori infection were used as the control.Result The percentage of CD4+CD25+ T cells of patients with H.pylori infection(35.09%) were higher than those of controls(23.94%).The percentage of CD4+CD25+ FOXP3+ subsets(19.01%) was higher than that in controls(13.09%).The level of serum TGF-β1 of patients with H.pylori infection(96.11 ng/mL) was higher than those of controls(51.49 ng/mL).With the H.pylori-associated gastric morbid progressing,the percentage of CD4+CD25+ T cells and the level of TGF-β1 increased with the development of disease.Conclusion With the H.pylori-associated gastric morbid progressing,the percentage of CD4+CD25+ T cells and the level of TGF-β1 increased with the development of disease.The percentage of CD4+CD25+ T cells and the level of TGF-β1 can serve as useful indicators in the determination of clinical stage.更多还原